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Bioinformatics Database on
"Radiosensitisers and Radioprotectors"

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Nigella sativa

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General Features                  Clinical Study                  Chemical Intervention                 Pharmacological Aspects                 
Radiobiological Aspects                  Biological Models                  Biological Target                  Toxicity                 


BIOLOGICAL MODELS

Test System Normal
tissue/tumor
Type of assay
/endpoint
Mice in vivo
Swiss albino mice free radical scavenging activity assay, LD50/30 assay[1]
Swiss female mice spleen, liver, bone marrow micronuclei assay, antioxidative enzyme assays, comet assay, TBARS assay[2]
female Swiss albino mice liver, spleen, intestines and brain, blood leukocytes TBARS assay, alkaline comet assay, antioxidative enzyme assay[3]
female Swiss albino mice bearing fibrosarcoma
rats in vivo
Sprague-Dawley rats tongue measurement of TAS and TOS, OSI, LOOH and -SH levels[4]
Sprague-Dawley rats lens biochemical analysis[5]
albino rats liver, kidney ultrastructure study[6]
male albino Wistar rats brain Nitric oxide synthase activity assay[7]
male Wistar albino rats spleen and thymus lymphoid follicles protein estimation, TBARS assay, histological study, leukocyte count, LD50/30 assay[8]
male Wistar albino rats peripheral blood lymphocytes ANAE staining, TBARS assay, determination of level nonenzymatic antioxidant markers[9]
Sprague-Dawley rats liver Measurement of TAS-TOS, -SH, and LOOH Levels and Calculation of OSI, enzyme assay[10]
Sprague-Dawley rats lens Chylack' cataract classification, enzyme assay[11]
male rats T lymphocytes,thymus TBARS assay, lipid profile analysis, flow cytometric analysis, apoptosis assay, histological study[12]
male CF rats intestine TBARS assay, antioxidative enzyme assay, intestinal morphological study[13]
In vitro study
mice ex vivo splenic lymphocytes TBARS assay, comet assay[2]
Plasmid pBR322 DNA Gel electrophoresis(plasmid relaxation study)[2]
REFERENCES:
1. Jagetia GC, Ravikiran PB, Radioprotective potential of Nigella Sativa extract in Swiss Albino Mice exposed to whole Body γ-radiation. Altern Integr Med 2014; 3(4):1-9.
http://dx.doi.org/10.4172/2327-5162.1000168
2. Rastogi L et l, Protection against radiation-induced oxidative damage by an ethanolic extract of Nigella sativa L. Int. J. Radiat. Biol. 2010;86(9):719–731.
http://dx.doi.org/10.3109/09553002.2010.484480
3. Velho-Pereira R et al, Radioprotection by macerated extract of Nigella sativa in normal tissues of fibrosarcoma bearing mice. Indian J Pharm Sci. 2012; 74(5): 403–414.
http://dx.doi.org/10.4103/0250474X. 108415
4. Üstün K et al, Radio-protective effects of Nigella sativa oil on oxidative stress in tongue tissue of rat. Oral Diseases 2014; 20(1):109–113.
http://dx.doi.org/10.1111/odi.12082
5. Taysi S et al, The radioprotective effect of Nigella sativa on nitrosative stress in lens tissue in radiation-induced cataract in rat. Cutan Ocul Toxicol. 2014; Early Online: 1–6.
http://dx.doi.org/10.3109/15569527.2014.910802
6. Abdel-Azeem MG, Evaluation of the radioprotective and curative role of a natural antioxidant against cellular ultrastructural hazards induced in rats by gamma radiation exposure. Isotope and Radiation Research. 2005; 37(6):1509-1523.
https://inis.iaea.org/search/search.aspx?orig_q=RN:37116890
7. Ahlatci A et al, Radiation-modifying abilities of Nigella sativa and Thymoquinone on radiation-induced nitrosative stress in the brain tissue. Phytomedicine 2014;21:740–744.
http://dx.doi.org/10.1016/j.phymed.2013.10.023
8. Assayed ME, Radioprotective effects of black seed (Nigella sativa) oil against hemopoietic damage and immunosuppression in gamma-irradiated rats. Immunopharmacology and Immunotoxicology 2010; 32(2): 284–296
http://dx.doi.org/10.3109/08923970903307552
9. Cemek M et al, In vivo radioprotective effects of Nigella sativa L oil and reduced glutathione against irradiation-induced oxidative injury and number of peripheral blood lymphocytes in rats. Photochemistry and Photobiology 2006; 82: 1691-1696.
http://dx.doi.org/10.156212006-06-15-RA-924
10. Cikman O et al, Radioprotective effects of Nigella sativa oil against oxidative stress in liver tissue of rats exposed to total head irradiation. Journal of Investigative Surgery 2014; 27: 262–266.
http://dx.doi.org/10.3109/08941939.2014.898811
11. Demir E et al, The effects of Nigella sativa oil, thymoquinone, propolis, and caffeic acid phenethyl ester on radiation-induced cataract. Wien Klin Wochenschr. 2015 Apr 10.
http://dx.doi.org/10.1007/s00508-015-0736-4
12. Guida MS et al, Thymoquinone rescues T Lymphocytes from gamma irradiation-induced apoptosis and exhaustion by modulating pro-inflammatory cytokine levels and PD-1, Bax, and Bcl-2 signaling. Cell Physiol Biochem 2016;38:786-800.
http://dx.doi.org/10.1159/00044303
13. Pandey N et al, Effect of methanolic fraction of the seeds of Nigella sativa Linn on radiation induced GI damage in rats. Nutr Food Technol. 2015; 1(1):1-5.
http://dx.doi.org/10.16966/2470-6086.102