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Bioinformatics Database on
"Radiosensitisers and Radioprotectors"

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Methotrexate

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General Features                  Clinical Study                  Chemical Intervention                 Pharmacological Aspects                 
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BIOLOGICAL MODELS

Test System Normal
tissue/tumor
Type of assay
/endpoint
rats in vivo
Female WAG/Rij rats change in blood-brain permiability[1]
Adult female Sprague-Dawley rats CNS forelimb paralysis analysis[2]
nude rats TE671 human rhabdomyosarcoma tumour xenograft percentage paralysis determination[3]
Sprague-Dawley rats brain, subependymal plate percentage mitotic count, percentage small dark nuclei count[4]
in vitro study
human in vitro breast cancer cells MDA-MB-231, 231-H2N, TrR1, TrR2 clonogenic survival assay[5], [6]
human in vitro HOS osteosarcoma cells Colony formation assay[7]
Nasal NK/T-cell lymphoma cell lines (Hank-1 and NK-92) MTT assay, annexin V and PI staining[8]
REFERENCES:
1. Storm AJ, van der Kogel AJ, Nooter K, Effect of X-irradiation on the pharmacokinetics of Methotrexate in rats: alteration of the blood-brain barrier. Eur J Cancer Clin Oncol. 1985 ;21(6):759-64.
http://dx.doi.org/10.1016/0277-5379(85)90275-5
2. Geyer JR et al, Radiation, methotrexate, and white matter necrosis: laboratory evidence for neural radioprotection with preirradiation methotrexate.Int J Radiat Oncol Biol Phys. 1988 ;15(2):373-5.
http://dx.doi.org/10.1016/S0360-3016(98)90018-9
3. Kassis AI et al, Therapeutic potential of 5-[125I]iodo-2’-deoxyuridine and methotrexate in the treatment of advanced neoplastic meningitis. Int J Radiat Biol 2004;80(11–12): 941–946.
http://dx.doi.org/10.1080/09553000400017671
4. Morris AD, Hopewell JW, Combined effects of radiation and methotrexate on the cells of the rat subependymal plate. J R Soc Med. 1983 ;76(10):848-52.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1439615/
5. Costantini DL et al, Methotrexate, Paclitaxel, and Doxorubicin radiosensitize HER2-Amplified human breast cancer cells to the Auger electron–emitting radiotherapeutic agent 111In-NLS-Trastuzumab. J Nucl Med 2010; 51:477–483.
http://dx.doi.org/10.2967/jnumed.109.069716
6. Costantini DL et al, Trastuzumab-resistant breast cancer cells remain sensitive to the Auger electron–emitting radiotherapeutic agent 111In-NLS-Trastuzumab and are radiosensitized by Methotrexate. J Nucl Med 2008; 49:1498–1505.
http://dx.doi.org/10.2967/jnumed.108.051771
7. Du L et al, Correlation of RAD51 and radiosensitization of methotrexate. Chinese Journal of Radiological Medicine and Protection 2012;32(1): 44-46.
https://inis.iaea.org/search/search.aspx?orig_q=RN:46111314
8. Kim A et al, A combination of methotrexate and irradiation promotes cell death in NK/T-cell lymphoma cells via down-regulation of NF-kB signaling. Leukemia Research 2012;36:350-357.
http://dx.doi.org/10.1016/j.leukres.2011.07.027