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Nigella sativa

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RADIOBIOLOGICAL ASPECTS

Dose
Modification
Ratio[DMF]:
Studies
related
to DMF :
N. sativa ethanolic seed extract is expected to exert its effects more prominently during early periods of irradiation.[1]
Concomintant N. sativa administration during radiotherapy can shoerten the period of radiation-related severe neutropenia, reduce morbidity secondary to bacterial and fungal infections, reduce the risk of cancer recurrence and micrometastasis[2]
Test System Dose of radiation Extract Conc. Time of extract administration DMF/qualitative
radiomodification
Reference
albino rats 8 Gy Nigella sativa oil, daily, at a dose of 30 mg / 100 g body weight by gavage, during 10 consecutive days before irradiation protection of liver and kidney from radiation damage [3]
after irradiation
Sprague-Dawley rats N. sativa oil, 1 g/kg/d, orally, for 10 days protection against radiation-induced cataract [4]
Thymoquinone, 50 mg/kg/d, IP daily starting 30 min before the dose radiation and subsequently daily for 10 days after irradiation
137Cs irradiation
male Wistar albino rats 4 Gy
0.667 Gy/min
Nigella sativa oil, 1 ml/kg body weight/ day, orally for 5 consecutive days, per week for 4 weeks before irradiation protection against immunosuppressive and oxidative effect of radiation [5]
60Co irradiation
male albino Wistar rats 5 Gy
0.49 Gy/min
Nigella sativa oil, 1 g/kg/day, continuied for 10 days through an orogastric tube starting 1 h before irradiation protection of brain against radiation-induced nitrosative stress [6]
Thymoquinone, 30 mg/kg/day, IP starting 30 min before radiation and continued for 10 days after irradiation
male Wistar albino rats 6 Gy
1.55 Gy/min
daily IP injection of 1 mL/kg Nigella sativa oil for 30 consecutive days before irradiation protection against radiation-induced oxidative injury [7]
Sprague-Dawley rats 5 Gy
0.49 Gy/min
1 g/kg/day, Nigella sativa oil, orally starting one hour before irradiation and continuing for 10 days protection of liver against radiation damage [8]
Sprague-Dawley rats 5 Gy
0.49 Gy/min
Nigella sativa oil, (1 g/kg/day) through an orogastric tube starting 1 h before irradiation dose and continuing for 10 days prevention of radiation-induced cataracts [9]
Thymoquinone (50 mg/kg/day) daily, IP starting 30 min before the radiation dose and subsequently daily for 10 days after irradiation
Swiss albino mice 10 Gy
1.33 Gy/min
Nigella sativa methanol seed extract, 25, 50, 100, 150 mg/kg, orally, for five consecutive days before irradiation Reduction in radiation-induced mortality [10]
Swiss albino mice 7, 8, 9, 10 or 11 Gy
1.33 Gy/min
Nigella sativa methanol seed extract, 150 mg/kg/day, orally, for five consecutive days before irradiation PF(survival LD50/30 up to 9.6 Gy) = 1.2 [10]
male CF rats 6 Gy Nigella sativa methanol seed extract, 200 mg/100 g bwt/day, orally 2 hrs before radiation and continued for 7th consecutive days protection of intestine against radiation damage [11]
Swiss female mice 2 Gy
0.32 Gy/min
Nigella sativa ethanol seed extract, 5–100 mg/kg bw, orally, for five consecutive days before irradiation protection against radiation-induced oxidative injury to spleen and liver [1]
7.5 Gy
0.32 Gy/min
increased survival
isolated mouse splenic lymphocytes 2 Gy Nigella sativa ethanol seed extract, 5–200 µg/ml for 1 h incubation before irradiation protection against radiation-induced lipid peroxidation and DNA damage [1]
Plasmid pBR322 DNA 200 Gy
3 Gy/ min
Nigella sativa ethanol seed extract, 10 µl before irradiation protection against radiation-induced DNA damage [1]
female Swiss albino mice 4 Gy
0.32 Gy/min
macerated extract of Nigella sativa seeds, 100 mg/kg, orally 1 h prior irradiation protection of liver, spleen, intestines and brain from radiation [12]
female Swiss albino mice bearing fibrosarcoma
Sprague-Dawley rats 5 Gy
0.49 Gy/min
N. sativa oil, 1g/kg/day, orally starting one hour before irradiation and continuing for 10 days protection against radiation-induced oxidative stress in tongue [13]
Fractionation study: Thymoquinone give orally (5 mg/kg), 1 hour after irradiation(4 Gy/14 days for 28 days) for 28 consecutive days, to male rats, protected T-lymphocytes from radiation-induced apoptosis[14]
REFERENCES
1. Rastogi L et l, Protection against radiation-induced oxidative damage by an ethanolic extract of Nigella sativa L. Int. J. Radiat. Biol. 2010;86(9):719–731.
http://dx.doi.org/10.3109/09553002.2010.484480
2. Chapter 3: Irradiation, Radioprotection and Nigella sativa, by Cemek M et al ; In : Herbal Radiomodulators: Applications in Medicine, Homeland Defence and Space, by Arora R, CAB International, 2008, Page no. 47.
https://books.google.co.in/books?id=lLdRCuTFdj0C&pg=PA47
3. Abdel-Azeem MG, Evaluation of the radioprotective and curative role of a natural antioxidant against cellular ultrastructural hazards induced in rats by gamma radiation exposure. Isotope and Radiation Research. 2005; 37(6):1509-1523.
https://inis.iaea.org/search/search.aspx?orig_q=RN:37116890
4. Taysi S et al, The radioprotective effect of Nigella sativa on nitrosative stress in lens tissue in radiation-induced cataract in rat. Cutan Ocul Toxicol. 2014; Early Online: 1–6.
http://dx.doi.org/10.3109/15569527.2014.910802
5. Assayed ME, Radioprotective effects of black seed (Nigella sativa) oil against hemopoietic damage and immunosuppression in gamma-irradiated rats. Immunopharmacology and Immunotoxicology 2010; 32(2): 284–296
http://dx.doi.org/10.3109/08923970903307552
6. Ahlatci A et al, Radiation-modifying abilities of Nigella sativa and Thymoquinone on radiation-induced nitrosative stress in the brain tissue. Phytomedicine 2014;21:740–744.
http://dx.doi.org/10.1016/j.phymed.2013.10.023
7. Cemek M et al, In vivo radioprotective effects of Nigella sativa L oil and reduced glutathione against irradiation-induced oxidative injury and number of peripheral blood lymphocytes in rats. Photochemistry and Photobiology 2006; 82: 1691-1696.
http://dx.doi.org/10.156212006-06-15-RA-924
8. Cikman O et al, Radioprotective effects of Nigella sativa oil against oxidative stress in liver tissue of rats exposed to total head irradiation. Journal of Investigative Surgery 2014; 27: 262–266.
http://dx.doi.org/10.3109/08941939.2014.898811
9. Demir E et al, The effects of Nigella sativa oil, thymoquinone, propolis, and caffeic acid phenethyl ester on radiation-induced cataract. Wien Klin Wochenschr. 2015 Apr 10.
http://dx.doi.org/10.1007/s00508-015-0736-4
10. Jagetia GC, Ravikiran PB, Radioprotective potential of Nigella Sativa extract in Swiss Albino Mice exposed to whole Body γ-radiation. Altern Integr Med 2014; 3(4):1-9.
http://dx.doi.org/10.4172/2327-5162.1000168
11. Pandey N et al, Effect of methanolic fraction of the seeds of Nigella sativa Linn on radiation induced GI damage in rats. Nutr Food Technol. 2015; 1(1):1-5.
http://dx.doi.org/10.16966/2470-6086.102
12. Velho-Pereira R et al, Radioprotection by macerated extract of Nigella sativa in normal tissues of fibrosarcoma bearing mice. Indian J Pharm Sci. 2012; 74(5): 403–414.
http://dx.doi.org/10.4103/0250474X. 108415
13. Üstün K et al, Radio-protective effects of Nigella sativa oil on oxidative stress in tongue tissue of rat. Oral Diseases 2014; 20(1):109–113.
http://dx.doi.org/10.1111/odi.12082
14. Guida MS et al, Thymoquinone rescues T Lymphocytes from gamma irradiation-induced apoptosis and exhaustion by modulating pro-inflammatory cytokine levels and PD-1, Bax, and Bcl-2 signaling. Cell Physiol Biochem 2016;38:786-800.
http://dx.doi.org/10.1159/00044303