| Action of Extract: |
Action on cell cycle:
MDA-MB-231 human breast cancer Cells exposed to low-dose methotrexate accumulated
in the G1/S phase of the cell cycle.[1]
|
| Target: |
Nucleic Acid
methotrexate disrupts DNA synthesis.[2]
proteins:
The
combination of radiation and methotrexate enhanced the inhibition of RAD51 expression in in HOS osteosarcoma cells.[3]
Combined treatment of radiation and methotrexate induced caspase activation.
and inhibited IkBα degradation in Nasal NK/T-cell lymphoma cells lines(Hank-1 and NK-92).[4]
Lipids:
sugars:
|
| Mechanism: |
Intracellular purine nucleotide
pool depletion by
methotrexate can indirectly affect the repair of DNA
damage in cells exposed to Auger electron radiation.[1]
Methotrexate is suggested to affect cell oxygenation
or alter the intracellular levels of endogenous radioprotectors
such as glutathione or other non-protein
thiols.[5]
Combined treatment
of Methotrexate and radiation significantly induced apoptosis and growth inhibition in both Nasal NK/T-cell lymphoma cells lines(Hank-1 and NK-92). The synergistic
cytotoxicity was correlated with blocking radiation-induced nuclear NF-kB and suppressing expression of NF-kB-mediated
anti-apoptotic proteins. [4]
|
| REFERENCES |
| 1. |
Costantini DL et al, Methotrexate, Paclitaxel, and Doxorubicin
radiosensitize HER2-Amplified human
breast cancer cells to the Auger
electron–emitting radiotherapeutic
agent 111In-NLS-Trastuzumab. J Nucl Med 2010; 51:477–483.
http://dx.doi.org/10.2967/jnumed.109.069716 |
| 2. |
Spittle MF, Methotrexate and radiation. Int J Radiat Oncol Biol Phys. 1978 ;4(1-2):103-7.
http://dx.doi.org/10.1016/0360-3016(78)90123-2
|
| 3. |
Du L et al, Correlation of RAD51 and radiosensitization of methotrexate. Chinese Journal of Radiological Medicine and Protection 2012;32(1): 44-46.
https://inis.iaea.org/search/search.aspx?orig_q=RN:46111314 |
| 4. |
Kim A et al, A combination of methotrexate and irradiation promotes cell death in NK/T-cell
lymphoma cells via down-regulation of NF-kB signaling. Leukemia Research 2012;36:350-357.
http://dx.doi.org/10.1016/j.leukres.2011.07.027 |
| 5. |
Geyer JR et al, Radiation, methotrexate, and white matter necrosis: laboratory evidence for neural radioprotection with preirradiation methotrexate.Int J Radiat Oncol Biol Phys. 1988 ;15(2):373-5.
http://dx.doi.org/10.1016/S0360-3016(98)90018-9
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